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svampe-amanita-smithiana

Dried basidiomata (0.1 g) were ground and suspended in 1 mL 50% watery methanol for 30 min. This raw extract was filtered through filter paper (Macherey–Nagel Mn 615¼) and 2 μL were spotted onto TLC plates (silica gel 60, Merck 105721) or HPTLC plates (high performance TLC plates; silica gel 60, Merck 105631). Chromatograms were developed using methanol–isopropanol–water–acetic acid–acetic acetate (5:8:12:15:40) as solvent system [ ]. The A.smithiana toxin was detected by spraying developed TLC plates with a ninhydrine solution, Ehrlich reagent, Morgan–Elson reagent or anisic aldehyde sulphuric acid according to [ ] ( ). As reference for the A.smithiana toxin, a raw extract of voucher material of A. smithiana [ ] was used because the toxin of A. smithiana is not fully determined and therefore no purified toxin is available. The toxin of A. smithiana can be detected at an Rf of 0.44 and stains orange–red with ninhydrine reagent, yellow with anisic aldehyde—sulphuric acid and yellow with Morgan–Elson reagent [ ].

[https://academic.oup.com/ndt/article/27/4/1380/1833136/Amanita-poisonings-resulting-in-acute-reversible]

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